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Brevinin-1VLa
General Information
DCTPep ID DCTPep02624
Peptide Name Brevinin-1VLa
Sequence FLGAIAGVAAKFLPKVFCFITKKC
Sequence Length 24
UniProt ID Not available
PubChem CID Not available
Origin Lithobates vaillanti
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HepG2 | Hepatoblastoma | LC50=4 µM | CytoTox 96 non-radiactive cytotoxicity assay | 1 h | 1 |
Hemolytic Activity Huµan erythrocytes: HC50=8 µM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep02624
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys18<--->Cys24
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C125H198N28O26S2
Absent amino acids DEHMNQRSWY
Theoretical pI 9.70
Acidic residues 0
Basic residues 4
Polar residues 5
Molecular weight (Average) 2573.24
Molecular weight (Monoisotopic) 2571.45
Common amino acids AFK
Net charge 4
Instability index (II) 10.53
Aliphatic index 105.83
Grand average of hydropathicity (GRAVY) 1.238
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.049, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 19379837
Title Purification of peptides with differential cytolytic activities from the skin secretions of the Central American frog, Lithobates vaillanti (Ranidae)
Doi 10.1016/j.cbpc.2009.04.003
Year 2009
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.