Peptide Libraray

Gradient3

General Information

DCTPep ID DCTPep02768

Peptide Name Gradient3

Sequence KVVDNFENILII

Sequence Length 12

UniProt ID Not available

PubChem CID Not available

Origin Synthetic

Type Synthetic peptide

Classification

Membrane-targeted

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
A549	Lung adenocarcinoma	EC50>50 µM	MTT assay	24 h	1
MCF-7	Invasive breast carcinoma of no special type	EC50>50 µM	MTT assay	24 h	1

Hemolytic Activity Not available

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure Not available

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C66H109N15O19

Absent amino acids ACGHMPQRSTWY

Theoretical pI 4.37

Acidic residues 2

Basic residues 1

Polar residues 2

Molecular weight (Average) 1416.68

Molecular weight (Monoisotopic) 1415.8

Common amino acids I

Net charge -1

Instability index (II) 29.71

Aliphatic index 178.33

Grand average of hydropathicity (GRAVY) 0.883

Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).

Extinction coefficients
Should not be visible by UV spectrophotometry.

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 31375699

Title In silico design and optimization of selective membranolytic anticancer peptides

Doi 10.1038/s41598-019-47568-9

Year 2019

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available