Peptide Libraray

AmphiArc2 Off8

General Information

DCTPep ID DCTPep02777

Peptide Name AmphiArc2 Off8

Sequence RFLKHFKTVYKRYWKVLGRL

Sequence Length 20

UniProt ID Not available

PubChem CID Not available

Origin Synthetic

Type Synthetic peptide

Classification

ACP Tumor active peptide Membrane-targeted

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
MCF-7	Invasive breast carcinoma of no special type	EC50=11.9 ± 0.1 µM	MTT assay	24 h	1
A549	Lung adenocarcinoma	EC50=17 ± 4 µM	MTT assay	24 h	1

Hemolytic Activity Human erythrocytes: HC50=144 ± 4 µM

Normal (non-cancerous) Cytotoxicity HDMEC: EC50=14.3 ± 0.2 µM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep02777

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C129H200N36O24

Absent amino acids ACDEIMNPQS

Theoretical pI 11.17

Acidic residues 0

Basic residues 8

Polar residues 4

Molecular weight (Average) 2639.23

Molecular weight (Monoisotopic) 2637.55

Common amino acids K

Net charge 8

Instability index (II) -8.11

Aliphatic index 87.50

Grand average of hydropathicity (GRAVY) -0.575

Half Life
1 hours (mammalian reticulocytes, in vitro).
2 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 3.213

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 31375699

Title In silico design and optimization of selective membranolytic anticancer peptides

Doi 10.1038/s41598-019-47568-9

Year 2019

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available

External Code

DBAASP ID DBAASPS_16602