AmphiArc2 Off2.7

General Information


DCTPep ID  DCTPep02786

Peptide Name   AmphiArc2 Off2.7

Sequence  RLIKRIKTWYRKAWKVVGKF

Sequence Length  20

UniProt ID  Not available

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Membrane-targeted



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
MCF-7 Invasive breast carcinoma of no special type EC50=11.2 ± 0.7 µM MTT assay 24 h 1
A549 Lung adenocarcinoma EC50=15 ± 1 µM MTT assay 24 h 1

Hemolytic Activity  Human erythrocytes: HC50>200 µM

Normal (non-cancerous) Cytotoxicity  HDMEC: EC50=23 ± 5 µM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep02786

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C125H202N36O23

Absent amino acids  CDEHMNPQS

Theoretical pI  11.76

Acidic residues  0

Basic residues  8

Polar residues  3

Molecular weight (Average)  2577.21

Molecular weight (Monoisotopic)  2575.57

Common amino acids  K

Net charge  8

Instability index (II)  -11.4

Aliphatic index  92.50

Grand average of hydropathicity (GRAVY)  -0.570

Half Life 
  1 hours (mammalian reticulocytes, in vitro).
  2 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 12490
  Abs 0.1% (=1 g/l) 4.846

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 31375699

Title  In silico design and optimization of selective membranolytic anticancer peptides

Doi 10.1038/s41598-019-47568-9

Year  2019

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPS_16611

DCTPep is developed by Dr.Zheng's team.