AmphiArc2 Off2.2.3
General Information
DCTPep ID DCTPep02792
Peptide Name AmphiArc2 Off2.2.3
Sequence HLLKKWRTWLRKAWHIVGKV
Sequence Length 20
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
Cell Line | Disease | Activity | Assay | Testing Time | Literature |
---|---|---|---|---|---|
MCF-7 | Invasive breast carcinoma of no special type | EC50=7 ± 1 µM | MTT assay | 24 h | 1 |
A549 | Lung adenocarcinoma | EC50=9 ± 1 µM | MTT assay | 24 h | 1 |
Hemolytic Activity Human erythrocytes: HC50=59 ± 6 µM
Normal (non-cancerous) Cytotoxicity HDMEC: EC50=6.1 ± 0.5 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep02792
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C124H195N37O22
Absent amino acids CDEFMNPQSY
Theoretical pI 12.03
Acidic residues 0
Basic residues 8
Polar residues 2
Molecular weight (Average) 2556.15
Molecular weight (Monoisotopic) 2554.53
Common amino acids K
Net charge 8
Instability index (II) 30.37
Aliphatic index 112.00
Grand average of hydropathicity (GRAVY) -0.435
Half Life
3.5 hours (mammalian reticulocytes, in vitro).
10 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 16500
Abs 0.1% (=1 g/l) 6.455
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 31375699
Title In silico design and optimization of selective membranolytic anticancer peptides
Doi 10.1038/s41598-019-47568-9
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPS_16617