ChBac3.4-NH2

General Information


DCTPep ID  DCTPep02801

Peptide Name   ChBac3.4-NH2

Sequence  RFRLPFRRPPIRIHPPPFYPPFRRFL

Sequence Length  26

UniProt ID  P85170 

PubChem CID  Not available

Origin  Capra hircus

Type  Native peptide

Classification

  

ACP Tumor active peptide Induce apoptosis



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
K562 Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia IC50=15.5 ± 0.3 μM MTT assay overnight 1
U-937 Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia IC50=8.1 ± 0.4 μM MTT assay overnight 1

Hemolytic Activity  Human erythrocytes: <5% Hemolysis=64 µM

Normal (non-cancerous) Cytotoxicity  Human PBMC: IC50=18.6 ± 0.8 µM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep02801

(Please note that there is the predicted structure, predicted by AlphaFold)

SequenceNo structure available

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C166H247N49O28

Absent amino acids  ACDEGKMNQSTVW

Theoretical pI  12.40

Acidic residues  0

Basic residues  8

Polar residues  1

Molecular weight (Average)  3377.1

Molecular weight (Monoisotopic)  3374.94

Common amino acids  P

Net charge  8

Instability index (II)  141.27

Aliphatic index  60.00

Grand average of hydropathicity (GRAVY)  -0.700

Half Life 
  1 hours (mammalian reticulocytes, in vitro).
  2 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 1490
  Abs 0.1% (=1 g/l) 0.441

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 33194795

Title  Caprine Bactenecins as Promising Tools for Developing New Antimicrobial and Antitumor Drugs

Doi 10.3389/fcimb.2020.552905

Year  2020

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPR_4306

DCTPep is developed by Dr.Zheng's team.