ChBac3.4-1-NH2

General Information


DCTPep ID  DCTPep02803

Peptide Name   ChBac3.4-1-NH2

Sequence  RFRLPFRRIHPPPFVRIHPPPFYRRFL

Sequence Length  27

UniProt ID  P85170 

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Induce apoptosis



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
U-937 Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia IC50=4.5 ± 1.6 μM MTT assay overnight 1
K562 Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia IC50=8.4 ± 1.1 μM MTT assay overnight 1

Hemolytic Activity  Human erythrocytes: <5% Hemolysis=64 µM

Normal (non-cancerous) Cytotoxicity  Human PBMC: IC50=12.1 ± 0.8 µM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep02803

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C172H256N52O29

Absent amino acids  ACDEGKMNQSTW

Theoretical pI  12.40

Acidic residues  0

Basic residues  9

Polar residues  1

Molecular weight (Average)  3516.26

Molecular weight (Monoisotopic)  3514.02

Common amino acids  PR

Net charge  9

Instability index (II)  121.94

Aliphatic index  68.52

Grand average of hydropathicity (GRAVY)  -0.578

Half Life 
  1 hours (mammalian reticulocytes, in vitro).
  2 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 1490
  Abs 0.1% (=1 g/l) 0.424

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 33194795

Title  Caprine Bactenecins as Promising Tools for Developing New Antimicrobial and Antitumor Drugs

Doi 10.3389/fcimb.2020.552905

Year  2020

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPS_16645

DCTPep is developed by Dr.Zheng's team.