Peptide Libraray

PuroA

General Information

DCTPep ID DCTPep02829

Peptide Name PuroA

Sequence FPVTWRWWKWWKG

Sequence Length 13

UniProt ID P33432

PubChem CID Not available

Origin Synthetic (Based Puroindoline-A)

Type Synthetic peptide

Classification

Membrane lysis Binding intracellular targets

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
HeLa	Human papillomavirus-related endocervical adenocarcinoma	IC50>250 μg/mL	MTT assay	24 h	1

Hemolytic Activity Sheep erythrocytes: No hemolysis up tp 500 µg/ml

Normal (non-cancerous) Cytotoxicity NIH 3T3: IC50=250 µg/ml

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep02829

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C98H123N23O15

Absent amino acids ACDEHILMNQSY

Theoretical pI 11.17

Acidic residues 0

Basic residues 3

Polar residues 2

Molecular weight (Average) 1863.2

Molecular weight (Monoisotopic) 1861.96

Common amino acids W

Net charge 3

Instability index (II) 58.3

Aliphatic index 22.31

Grand average of hydropathicity (GRAVY) -0.962

Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 27500
Abs 0.1% (=1 g/l) 14.760

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 33207639

Title Effects of Rationally Designed Physico-Chemical Variants of the Peptide PuroA on Biocidal Activity towards Bacterial and Mammalian Cells

Doi 10.3390/ijms21228624

Year 2020

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available