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TRPs R8
General Information
DCTPep ID DCTPep02832
Peptide Name TRPs R8
Sequence RRRRWRWWRWWRR
Sequence Length 13
UniProt ID P33432
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide Membrane lysis Binding intracellular targets
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50=250 μg/mL | MTT assay | 24 h | 1 |
Hemolytic Activity Sheep erythrocytes: No hemolysis up tp 500 µg/ml
Normal (non-cancerous) Cytotoxicity NIH 3T3: IC50>250 µg/ml
Target Not available
Affinity Not available
Mechanism Dual mechanism, with the disruption of the membrane integrity possibly being the main cause of cell death, but only after peptide translocation and binding to intracellular targets.
Structure Information
PDB ID Not available
Predicted Structure DCTPep02832
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C103H148N42O14
Absent amino acids ACDEFGHIKLMNPQSTVY
Theoretical pI 12.85
Acidic residues 0
Basic residues 8
Polar residues 0
Molecular weight (Average) 2198.58
Molecular weight (Monoisotopic) 2197.22
Common amino acids R
Net charge 8
Instability index (II) 317.66
Aliphatic index 0.00
Grand average of hydropathicity (GRAVY) -3.115
Half Life
1 hours (mammalian reticulocytes, in vitro).
2 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 27500
Abs 0.1% (=1 g/l) 12.508
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 33207639
Title Effects of Rationally Designed Physico-Chemical Variants of the Peptide PuroA on Biocidal Activity towards Bacterial and Mammalian Cells
Year 2020
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPS_16983
This work is licensed under a Creative Commons Attribution 4.0 International License.