Arenicin-3 [Y5N,Y9R,Y17H], NZ17143

General Information


DCTPep ID  DCTPep02851

Peptide Name   Arenicin-3 [Y5N,Y9R,Y17H], NZ17143

Sequence  GFCWNVCVRRNGVRVCHRRCN

Sequence Length  21

UniProt ID  A0A3F2YLM5 

PubChem CID  Not available

Origin  Synthetic (Derived from Arenicin-3)

Type  Synthetic peptide

Classification

  

Membrane-targeted



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HepG2 Hepatoblastoma CC50>300 μg/ml Resazurin assay 24 h 1

Hemolytic Activity  Human erythrocytes: HC10= µg/ml

Normal (non-cancerous) Cytotoxicity  HEK-293: CC50>300 µg/ml; HK-2: CC50>250 µg/ml

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep02851

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys3<--->Cys20; Cys7<--->Cys16

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C104H168N42O25S4

Absent amino acids  ADEIKLMPQSTY

Theoretical pI  10.45

Acidic residues  0

Basic residues  6

Polar residues  9

Molecular weight (Average)  2534.98

Molecular weight (Monoisotopic)  2533.2

Common amino acids  R

Net charge  6

Instability index (II)  91.88

Aliphatic index  55.24

Grand average of hydropathicity (GRAVY)  -0.395

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 5750
  Abs 0.1% (=1 g/l) 2.268, assuming all pairs of Cys residues form cystines
  Ext. coefficient 5500
  Abs 0.1% (=1 g/l) 2.170, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 32576824

Title  An amphipathic peptide with antibiotic activity against multidrug-resistant Gram-negative bacteria

Doi 10.1038/s41467-020-16950-x

Year  2020

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPS_17221

DCTPep is developed by Dr.Zheng's team.