H-10, Hymenochirin-1B [E6,N10,A18,A22 S5]

General Information


DCTPep ID  DCTPep02891

Peptide Name   H-10, Hymenochirin-1B [E6,N10,A18,A22 S5]

Sequence  IKLSPⓍTKDⓍLKKVLKGⓍIKGⓍIAVAKMV

Sequence Length  29

UniProt ID  W8PRC4 

PubChem CID  Not available

Origin  Synthetic (Derived from Hymenochirin-1B)

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Stapled Peptides



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HepG2 Hepatoblastoma IC50=1.50±0.21 μM MTT assay 96 h 1
HCT 116 Colon carcinoma IC50=3.51±0.37 μM MTT assay 96 h 1
A549 Lung adenocarcinoma IC50=3.59±0.12 μM MTT assay 96 h 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  Not available

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Stapled: Ⓧ(6) and Ⓧ (10), Ⓧ(18) and Ⓧ (22) are cross-linked by hydrocarbon stapling

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  Ⓧ=(S)-N-Fmoc-2-(4′-pentenyl)alanine

Chiral  L



Physicochemical Information


Formula  Not Applicable

Absent amino acids  Not Applicable

Theoretical pI  Not Applicable

Acidic residues  Not Applicable

Basic residues  Not Applicable

Polar residues  Not Applicable

Molecular weight (Average)  Not Applicable

Molecular weight (Monoisotopic)  Not Applicable

Common amino acids  Not Applicable

Net charge  Not Applicable

Instability index (II)  Not Applicable

Aliphatic index  Not Applicable

Grand average of hydropathicity (GRAVY)  Not Applicable

Half Life 
  Not Applicable

Extinction coefficients 
  Not Applicable

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 30789695

Title  Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy

Doi 10.1021/acschembio.9b00046

Year  2019

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPS_18014

DCTPep is developed by Dr.Zheng's team.