sC18 [R10F,E15F]

General Information


DCTPep ID  DCTPep02910

Peptide Name   sC18 [R10F,E15F]

Sequence  GLEKRKRKFFNKIKFK

Sequence Length  16

UniProt ID  P25230 

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Cell-penetrating peptides Membrane-targeted



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
MCF-7 Invasive breast carcinoma of no special type 60% Killing=40 µM Resazurin assay 24 h 1
HeLa Human papillomavirus-related endocervical adenocarcinoma 75% Killing=40 µM Resazurin assay 24 h 1

Hemolytic Activity  Human erythrocytes: <10% Hemolysis =40 µM

Normal (non-cancerous) Cytotoxicity  HEK293: 20% Killing=40 µM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep02910

(Please note that there is the predicted structure, predicted by AlphaFold)

SequenceNo structure available

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C98H163N29O20

Absent amino acids  ACDHMPQSTVWY

Theoretical pI  11.26

Acidic residues  1

Basic residues  8

Polar residues  2

Molecular weight (Average)  2067.55

Molecular weight (Monoisotopic)  2066.26

Common amino acids  K

Net charge  7

Instability index (II)  44.92

Aliphatic index  48.75

Grand average of hydropathicity (GRAVY)  -1.444

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Should not be visible by UV spectrophotometry.

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 33313751

Title  Multistep optimization of a cell-penetrating peptide towards its antimicrobial activity

Doi 10.1042/BCJ20200698

Year  2021

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPS_18143

DCTPep is developed by Dr.Zheng's team.