Peptide Libraray

Puma-BH3

General Information

DCTPep ID DCTPep03025

Peptide Name Puma-BH3

Sequence EQWAREIGAQLRRMADDLNA

Sequence Length 20

UniProt ID Not available

PubChem CID Not available

Origin Synthetic

Type Synthetic peptide

Classification

ACP Tumor active peptide Cancer targeted peptides Induce apoptosis Bcl-xL inhibitors BH3 peptides and mimetics

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
MDA-MB-231 (Bc1-xL dependent)	Breast adenocarcinoma	EC50=1.006 ± 0.337 μM	Not available	Not available	1
OCI-Ly1 (Bcl-2 dependent)	Diffuse large B-cell lymphoma	EC50=1.967 ± 0.48 μM	Not available	Not available	1

Hemolytic Activity Not available

Normal (non-cancerous) Cytotoxicity Not available

Target Bcl-xL

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep03025

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C98H159N33O32S1

Absent amino acids CFHKPSTVY

Theoretical pI 4.78

Acidic residues 4

Basic residues 3

Polar residues 2

Molecular weight (Average) 2343.6

Molecular weight (Monoisotopic) 2342.15

Common amino acids A

Net charge -1

Instability index (II) 57.2

Aliphatic index 78.50

Grand average of hydropathicity (GRAVY) -0.905

Half Life
1 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.347

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 25451027

Title Potent and specific peptide inhibitors of human pro-survival protein Bcl-xL

Doi 10.1016/j.jmb.2014.09.030

Year 2015

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available