B1-L-4

General Information


DCTPep ID  DCTPep05884

Peptide Name   B1-L-4

Sequence  VKRFKKFFⓍKLKⓍLV

Sequence Length  15

UniProt ID  Not available

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Cancer targeted peptides Stapled Peptides Membrane-targeted



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
Huh-7 Adult hepatocellular carcinoma; Adult hepatocellular carcinoma IC50=13.41±1.92 μM CCK-8 assay 48 h 1
MCF-7 Invasive breast carcinoma of no special type IC50=15.20±1.02 μM CCK-8 assay 48 h 1
U87 Glioblastoma IC50=17.91±3.23 μM CCK-8 assay 48 h 1

Hemolytic Activity  HC50>80 μM

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  Not available

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  35

Linear/Cyclic  Cyclic (Stapled)

Disulfide/Other Bond  Stapled: Ⓧ are cross-linked by hydrocarbon stapling

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  Ⓧ=(S)-2-(4-pentenyl) alanine amino acid

Chiral  L



Physicochemical Information


Formula  Not Applicable

Absent amino acids  Not Applicable

Theoretical pI  Not Applicable

Acidic residues  Not Applicable

Basic residues  Not Applicable

Polar residues  Not Applicable

Molecular weight (Average)  Not Applicable

Molecular weight (Monoisotopic)  Not Applicable

Common amino acids  Not Applicable

Net charge  Not Applicable

Instability index (II)  Not Applicable

Aliphatic index  Not Applicable

Grand average of hydropathicity (GRAVY)  Not Applicable

Half Life 
  Not Applicable

Extinction coefficients 
  Not Applicable

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 35464194

Title  Design, Synthesis, and Antitumor Activity Study of All-Hydrocarbon-Stapled B1-Leu Peptides

Doi 10.3389/fchem.2022.840131

Year  2022

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.