SPMI-HIF2-3

General Information


DCTPep ID  DCTPep05916

Peptide Name   SPMI-HIF2-3

Sequence  TSFⓍEYWALLⓍXLAXYXP

Sequence Length  18

UniProt ID  Not available

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Cancer targeted peptides Stapled Peptides Induce apoptosis Induce cell cycle arrest MDM6/MDMX Inhibitor



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HCT 116 p53+/+ Colon carcinoma IC50=15.2 μM CCK-8 assay 72 h 1
HCT 116 p53-/- Colon carcinoma IC50>100 μM CCK-8 assay 72 h 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Not available

Target  MDM2, MDMX

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  Not available

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  24

Linear/Cyclic  Cyclic (Stapled)

Disulfide/Other Bond  Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling

N-terminal Modification  Acetylation

C-terminal Modification  Amidation

Other Modification  Ⓧ(4)=R-octenyl-alanine; Ⓧ(11)=S-pentenyl-alanine; X(12)=Peg3, polyethylene glycol; X(15)=Hyp, hydroxyproline; X(17)=Hle, homoleucine

Chiral  L



Physicochemical Information


Formula  Not Applicable

Absent amino acids  Not Applicable

Theoretical pI  Not Applicable

Acidic residues  Not Applicable

Basic residues  Not Applicable

Polar residues  Not Applicable

Molecular weight (Average)  Not Applicable

Molecular weight (Monoisotopic)  Not Applicable

Common amino acids  Not Applicable

Net charge  Not Applicable

Instability index (II)  Not Applicable

Aliphatic index  Not Applicable

Grand average of hydropathicity (GRAVY)  Not Applicable

Half Life 
  Not Applicable

Extinction coefficients 
  Not Applicable

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 36185610

Title  Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression

Doi 10.7150/thno.75444

Year  2022

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.