P1

General Information


DCTPep ID  DCTPep06831

Peptide Name   P1

Sequence  ANGRGD

Sequence Length  6

UniProt ID  Not available

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Membrane lysis Induce apoptosis



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HepG2 Hepatoblastoma IC50=11.53µg/mL MTT assay 48h 1
MCF-7 Invasive breast carcinoma of no special type IC50=15.73µg/mL MTT assay 48h 1
HEp-2 Human papillomavirus-related endocervical adenocarcinoma IC50=17.84µg/mL MTT assay 48h 1
A375 Amelanotic Amelanotic melanoma IC50=69.87µg/mL MTT assay 48h 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Vero: IC50=71.37µg/mL

Target  Not available

Affinity  Not available

Mechanism  The perceived cancer cell-selective killing of both peptides P1 and P2 seems to stalk from cationic peptides and the electrostatic interactions between the anionic lipids of cancer cells on account of specific binding between integrins proteins and N (APN/CD13) ligand-receptor on the cancer cell membrane and the presence of binding RGD and NGR motifs in peptides.



Structure Information


PDB ID  Not available

Predicted Structure  Not available

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  1-oxo-1H-phenalene-2,3-carbonitrile (Ar)

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  Not Applicable

Absent amino acids  Not Applicable

Theoretical pI  Not Applicable

Acidic residues  Not Applicable

Basic residues  Not Applicable

Polar residues  Not Applicable

Molecular weight (Average)  588.58

Molecular weight (Monoisotopic)  588.26

Common amino acids  Not Applicable

Net charge  Not Applicable

Instability index (II)  Not Applicable

Aliphatic index  Not Applicable

Grand average of hydropathicity (GRAVY)  Not Applicable

Half Life 
  Not Applicable

Extinction coefficients 
  Not Applicable

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 36863075

Title  Design and synthesis of novel N-terminal peptides of integrin and aminopeptidase are new finding for anticancer activity

Doi 10.1016/j.bioorg.2023.106434

Year  2023

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.