This work is licensed under a Creative Commons Attribution 4.0 International License.
B1AW
General Information
DCTPep ID DCTPep06874
Peptide Name B1AW
Sequence FLPLLAGLAANFLPQIICKIARKC
Sequence Length 24
UniProt ID Not available
PubChem CID Not available
Origin Skin secretions of the Wuyi torrent frog, Amolops wuyiensis (A. wuyiensisi)
Type Native peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| H838 | Human non-small cell lung cancer cells | IC50=32.15µM | MTT assay | 24h | 1 |
| PC-3 | Prostate carcinoma | IC50=33.46µM | MTT assay | 24h | 1 |
| U251MG | Human glioblastoma/astrocytoma cancer cells | IC50=33.56µM | MTT assay | 24h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity HMEC-1: IC50=32.96μM; HaCaT: IC50=44.09μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure Not available
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C123H205N31O27S2
Absent amino acids DEHMSTVWY
Theoretical pI 9.50
Acidic residues 0
Basic residues 3
Polar residues 4
Molecular weight (Average) 2614.29
Molecular weight (Monoisotopic) 2612.51
Common amino acids L
Net charge 3
Instability index (II) 23.86
Aliphatic index 146.67
Grand average of hydropathicity (GRAVY) 1.142
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.048, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 37228702
Title Discovery and analysis of a novel antimicrobial peptide B1AW from the skin secretion of Amolops wuyiensis and improving the membrane-binding affinity through the construction of the lysine-introduced analogue
Doi 10.1016/j.csbj.2023.05.006
Year 2023
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.