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B1AW-K
General Information
DCTPep ID DCTPep06875
Peptide Name B1AW-K
Sequence FLPLLAGLAANFLPKIICKIARKC
Sequence Length 24
UniProt ID Not available
PubChem CID Not available
Origin Synthetic (derived from B1AW)
Type Synthetic peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| U251MG | Human glioblastoma/astrocytoma cancer cells | IC50=3.86µM | MTT assay | 24h | 1 |
| PC-3 | Prostate carcinoma | IC50=4.283µM | MTT assay | 24h | 1 |
| H838 | Human non-small cell lung cancer cells | IC50=5.15µM | MTT assay | 24h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity HMEC-1: IC50=26.45μM; HaCaT: IC50=20.53μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure Not available
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C124H209N31O26S2
Absent amino acids DEHMQSTVWY
Theoretical pI 9.85
Acidic residues 0
Basic residues 4
Polar residues 4
Molecular weight (Average) 2614.34
Molecular weight (Monoisotopic) 2612.54
Common amino acids L
Net charge 4
Instability index (II) 12.3
Aliphatic index 146.67
Grand average of hydropathicity (GRAVY) 1.125
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.048, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 37228702
Title Discovery and analysis of a novel antimicrobial peptide B1AW from the skin secretion of Amolops wuyiensis and improving the membrane-binding affinity through the construction of the lysine-introduced analogue
Doi 10.1016/j.csbj.2023.05.006
Year 2023
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.