V1

General Information


DCTPep ID  DCTPep00112

Peptide Name   V1

Sequence  CVKVKVKVGSGVKVKVKVC

Sequence Length  19

UniProt ID  Not available

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Membrane-targeted



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
THP-1 Childhood acute monocytic leukemia; Acute monoblastic/monocytic leukemia IC50=45 µM CellTiter 96 AQueous One Solution assay 1 h 1

Hemolytic Activity  Human erythrocytes: 50% Hemolysis=880 µM

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00112

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys1<--->Cys19

N-terminal Modification  Acetylization

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C89H167N25O21S2

Absent amino acids  ADEFHILMNPQRTWY

Theoretical pI  10.05

Acidic residues  0

Basic residues  6

Polar residues  5

Molecular weight (Average)  1987.58

Molecular weight (Monoisotopic)  1986.22

Common amino acids  V

Net charge  6

Instability index (II)  -34.87

Aliphatic index  122.11

Grand average of hydropathicity (GRAVY)  0.716

Half Life 
  1.2 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 125
  Abs 0.1% (=1 g/l) 0.063, assuming all pairs of Cys residues form cystines
  Ext. coefficient 0
  Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 15328096

Title  De novo design of potent antimicrobial peptides

Doi 10.1128/AAC.48.9.3349-3357.2004

Year  2004

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.