This work is licensed under a Creative Commons Attribution 4.0 International License.
Brevinin-1DYb
General Information
DCTPep ID DCTPep00149
Peptide Name Brevinin-1DYb
Sequence FLSLALAALPKLFCLIFKKC
Sequence Length 20
UniProt ID Not available
PubChem CID Not available
Origin Rana dybowskii; Rana pirica; Rana amurensis
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| RT-4(G2) | Prostate tumor | IC50=135.3 µM | Cell Viability assay | 48 h | 1 |
| 486P | Bladder carcinoma | IC50=28.6 µM | LDH leakage assay | 48 h | 1 |
| 647V | Bladder carcinoma | IC50=31 µM | Cell Viability assay | 48 h | 1 |
| RT-4(G2) | Prostate tumor | IC50=32.3 µM | LDH leakage assay | 48 h | 1 |
| NCI-H157 | Lung squamous cell carcinoma | IC50=4.5 µM | MTT assay | Not available | 1 |
| U-251MG | Astrocytoma | IC50=4.6 µM | MTT assay | Not available | 1 |
| RT-4(G2) | Prostate tumor | IC50=484 µM | WST-1 assay | 48 h | 1 |
| 647V | Bladder carcinoma | IC50=52.4 µM | WST-1 assay | 48 h | 1 |
| 647V | Bladder carcinoma | IC50=54.1 µM | LDH leakage assay | 48 h | 1 |
| 486P | Bladder carcinoma | IC50=57.8 µM | WST-1 assay | 48 h | 1 |
| 486P | Bladder carcinoma | IC50=59.4 µM | Cell Viability assay | 48 h | 1 |
| PC-3 | Prostate carcinoma | IC50=6.4 µM | MTT assay | Not available | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=9.1 µM | MTT assay | Not available | 1 |
| MDA-MB-435S | Amelanotic melanoma | IC50=9.5 µM | MTT assay | Not available | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis<5 µM; 50% Hemolysis=125 µM; 50% Hemolysis=7 µM; Horse erythrocytes: 50% Hemolysis=4.7 µM
Normal (non-cancerous) Cytotoxicity HMEC-1: IC50=25.2 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00149
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys14<--->Cys20
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C110H179N23O22S2
Absent amino acids DEGHMNQRTVWY
Theoretical pI 9.39
Acidic residues 0
Basic residues 3
Polar residues 3
Molecular weight (Average) 2239.89
Molecular weight (Monoisotopic) 2238.3
Common amino acids L
Net charge 3
Instability index (II) 17
Aliphatic index 151.50
Grand average of hydropathicity (GRAVY) 1.600
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.056, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 29366784
Title Identification of novel Amurin-2 variants from the skin secretion of Rana amurensis, and the design of cationicity-enhanced analogues
Doi 10.1016/j.bbrc.2018.01.124
Year 2018
Literature 2
Pubmed ID 17688900
Title Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog, Rana dybowskii
Doi 10.1016/j.toxicon.2007.06.023
Year 2007
Literature 3
Pubmed ID 19539775
Title Characterization of antimicrobial peptides isolated from the skin of the Chinese frog, Rana dybowskii
Doi 10.1016/j.cbpb.2009.05.015
Year 2009
Literature 4
Pubmed ID 15003829
Title A family of brevinin-2 peptides with potent activity against Pseudomonas aeruginosa from the skin of the Hokkaido frog, Rana pirica
Doi 10.1016/j.regpep.2003.12.003
Year 2004
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.