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Brevinin-1BLc
General Information
DCTPep ID DCTPep00206
Peptide Name Brevinin-1BLc
Sequence FLPIIAGIAAKFLPKIFCTISKKC
Sequence Length 24
UniProt ID B6CPR0
PubChem CID Not available
Origin North America, leopard frog, Lithobates blairi
Type Native peptide
Classification
ACP Tumor active peptide Membrane lysis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| Hep-G2 | Hepatoblastoma; Hepatoblastoma | LD50=6 μM | Cytolytic assays | Not available | 1 |
Hemolytic Activity Human erythrocytes: LD50=9 μM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism potent cytolytic activity
Structure Information
PDB ID Not available
Predicted Structure DCTPep00206
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Cys18<--->Cys24
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C127H208N28O27S2
Absent amino acids DEHMNQRVWY
Theoretical pI 9.70
Acidic residues 0
Basic residues 4
Polar residues 5
Molecular weight (Average) 2623.34
Molecular weight (Monoisotopic) 2621.52
Common amino acids I
Net charge 4
Instability index (II) 32.14
Aliphatic index 126.25
Grand average of hydropathicity (GRAVY) 1.175
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.048, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 19254736
Title Peptides with potent cytolytic activity from the skin secretions of the North American leopard frogs, Lithobates blairi and Lithobates yavapaiensis
Doi 10.1016/j.toxicon.2009.02.018
Year 2009
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_4214
This work is licensed under a Creative Commons Attribution 4.0 International License.