Brevinin-1Ya

General Information


DCTPep ID  DCTPep00207

Peptide Name   Brevinin-1Ya

Sequence  FLPVIAGVAANFLPKLFCAISKKC

Sequence Length  24

UniProt ID  Not available

PubChem CID  Not available

Origin  North America, leopard frog, Lithobates blairi

Type  Native peptide

Classification

  

ACP Tumor active peptide Membrane lysis



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
Hep-G2 Hepatoblastoma; Hepatoblastoma LD50=16 μM Cytolytic assays Not available 1

Hemolytic Activity  Human erythrocytes: LD50=18 μM

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  potent cytolytic activity



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00207

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Cys18<--->Cys24

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C122H196N28O27S2

Absent amino acids  DEHMQRTWY

Theoretical pI  9.39

Acidic residues  0

Basic residues  3

Polar residues  5

Molecular weight (Average)  2551.19

Molecular weight (Monoisotopic)  2549.43

Common amino acids  A

Net charge  3

Instability index (II)  23.86

Aliphatic index  122.08

Grand average of hydropathicity (GRAVY)  1.242

Half Life 
  1.1 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 125
  Abs 0.1% (=1 g/l) 0.049, assuming all pairs of Cys residues form cystines
  Ext. coefficient 0
  Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 19254736

Title  Peptides with potent cytolytic activity from the skin secretions of the North American leopard frogs, Lithobates blairi and Lithobates yavapaiensis

Doi 10.1016/j.toxicon.2009.02.018

Year  2009

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPR_4215

DCTPep is developed by Dr.Zheng's team.