RGD-La

General Information


DCTPep ID  DCTPep00406

Peptide Name   RGD-La

Sequence  RGDLLRHVVKILEKYL

Sequence Length  16

UniProt ID  Not available

PubChem CID  Not available

Origin  Arg-Gly-Asp(RGD) tripeptide ana temporins

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Membrane-targeted



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
MEC Cholangiocarcinoma; Cholangiocarcinoma 19.76 Inhibition ratio at 50 µg/ml MTT assay 24 h 1
HL-7702(L-02) Human papillomavirus-related endocervical adenocarcinoma 36.25 Inhibition ratio at 50 µg/ml MTT assay 24 h 1
A549 Lung adenocarcinoma 47.26Inhibition ratio at 50 µg/ml MTT assay 24 h 1
Hep-G2 Hepatoblastoma; Hepatoblastoma 60.22 Inhibition ratio at 50 µg/ml MTT assay 24 h 1
BGC-823 Human papillomavirus-related endocervical adenocarcinoma 62.49 Inhibition ratio at 50 µg/ml MTT assay 24 h 1
SMMC-7721 Human papillomavirus-related endocervical adenocarcinoma 70.39 Inhibition ratio at 50 µg/ml MTT assay 24 h 1
SW1116 Colon adenocarcinoma 72.69 Inhibition ratio at 50 µg/ml MTT assay 24 h 1
HeLa Human papillomavirus-related endocervical adenocarcinoma 73.09 Inhibition ratio at 50 µg/ml MTT assay 24 h 1

Hemolytic Activity  Showed no hemolytic activity against rabbit erythrocytes and human erythrocytes at 250 µg/ml

Normal (non-cancerous) Cytotoxicity  Showed little effects on the proliferation of the 293T human embryonic kidney cell line, MECs, and human lymphomonocytes at concentrations of less than 50 µg/ml

Target  Not available

Affinity  Not available

Mechanism  A membrane-disturbing action seems to be the major mechanism for cell death



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00406

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C90H154N26O22

Absent amino acids  ACFMNPQSTW

Theoretical pI  9.70

Acidic residues  2

Basic residues  5

Polar residues  2

Molecular weight (Average)  1952.37

Molecular weight (Monoisotopic)  1951.17

Common amino acids  L

Net charge  3

Instability index (II)  33.08

Aliphatic index  158.12

Grand average of hydropathicity (GRAVY)  -0.037

Half Life 
  1 hours (mammalian reticulocytes, in vitro).
  2 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 1490
  Abs 0.1% (=1 g/l) 0.763

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 22641352

Title  Designed synthetic analogs of the α-helical peptide temporin-La with improved antitumor efficacies via charge modification and incorporation of the integrin αvβ3 homing domain

Doi 10.1002/psc.2420

Year  2012

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.