Peptide Libraray

RGD-La

General Information

DCTPep ID DCTPep00406

Peptide Name RGD-La

Sequence RGDLLRHVVKILEKYL

Sequence Length 16

UniProt ID Not available

PubChem CID Not available

Origin Arg-Gly-Asp(RGD) tripeptide ana temporins

Type Synthetic peptide

Classification

ACP Tumor active peptide Membrane-targeted

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
MEC	Cholangiocarcinoma; Cholangiocarcinoma	19.76 Inhibition ratio at 50 µg/ml	MTT assay	24 h	1
HL-7702(L-02)	Human papillomavirus-related endocervical adenocarcinoma	36.25 Inhibition ratio at 50 µg/ml	MTT assay	24 h	1
A549	Lung adenocarcinoma	47.26Inhibition ratio at 50 µg/ml	MTT assay	24 h	1
Hep-G2	Hepatoblastoma; Hepatoblastoma	60.22 Inhibition ratio at 50 µg/ml	MTT assay	24 h	1
BGC-823	Human papillomavirus-related endocervical adenocarcinoma	62.49 Inhibition ratio at 50 µg/ml	MTT assay	24 h	1
SMMC-7721	Human papillomavirus-related endocervical adenocarcinoma	70.39 Inhibition ratio at 50 µg/ml	MTT assay	24 h	1
SW1116	Colon adenocarcinoma	72.69 Inhibition ratio at 50 µg/ml	MTT assay	24 h	1
HeLa	Human papillomavirus-related endocervical adenocarcinoma	73.09 Inhibition ratio at 50 µg/ml	MTT assay	24 h	1

Hemolytic Activity Showed no hemolytic activity against rabbit erythrocytes and human erythrocytes at 250 µg/ml

Normal (non-cancerous) Cytotoxicity Showed little effects on the proliferation of the 293T human embryonic kidney cell line, MECs, and human lymphomonocytes at concentrations of less than 50 µg/ml

Target Not available

Affinity Not available

Mechanism A membrane-disturbing action seems to be the major mechanism for cell death

Structure Information

PDB ID Not available

Predicted Structure DCTPep00406

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C90H154N26O22

Absent amino acids ACFMNPQSTW

Theoretical pI 9.70

Acidic residues 2

Basic residues 5

Polar residues 2

Molecular weight (Average) 1952.37

Molecular weight (Monoisotopic) 1951.17

Common amino acids L

Net charge 3

Instability index (II) 33.08

Aliphatic index 158.12

Grand average of hydropathicity (GRAVY) -0.037

Half Life
1 hours (mammalian reticulocytes, in vitro).
2 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 0.763

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 22641352

Title Designed synthetic analogs of the α-helical peptide temporin-La with improved antitumor efficacies via charge modification and incorporation of the integrin αvβ3 homing domain

Doi 10.1002/psc.2420

Year 2012

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available