This work is licensed under a Creative Commons Attribution 4.0 International License.
HPRP-A1-TAT
General Information
DCTPep ID DCTPep00578
Peptide Name HPRP-A1-TAT
Sequence FKKLKKLFSKLWNWKRKKRRQRRR
Sequence Length 24
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50=1.8±0.02 μM | MTT assay | 24h | 1 |
| B16 | Mouse melanoma | IC50=3.5±0.03 μM | MTT assay | 24h | 1 |
| SGC-7901 | Human papillomavirus-related endocervical adenocarcinoma | IC50=4.8±0.08 μM | MTT assay | 24h | 1 |
| Hep-G2 | Hepatoblastoma; Hepatoblastoma | IC50=5.8±0.36 μM | MTT assay | 24h | 1 |
Hemolytic Activity Human RBC: MHC>500 μM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00578
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Acetylation
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C154H260N54O28
Absent amino acids ACDEGHIMPTVY
Theoretical pI 12.71
Acidic residues 0
Basic residues 14
Polar residues 2
Molecular weight (Average) 3316.1
Molecular weight (Monoisotopic) 3314.06
Common amino acids K
Net charge 14
Instability index (II) 106.65
Aliphatic index 48.75
Grand average of hydropathicity (GRAVY) -2.117
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 11000
Abs 0.1% (=1 g/l) 3.317
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 26405806
Title TAT Modification of Alpha-Helical Anticancer Peptides to Improve Specificity and Efficacy
Doi 10.1371/journal.pone.0138911
Year 2015
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.