Ctn[1-14]

General Information


DCTPep ID  DCTPep00581

Peptide Name   Ctn[1-14]

Sequence  KRFKKFFKKVKKSV

Sequence Length  14

UniProt ID  Not available

PubChem CID  Not available

Origin  Rattlesnake Venom Cathelicidin

Type  Native peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HeLa S3 Breast cancer IC50=1 µM Resazurin assay 4h 1
THP-1 Childhood acute monocytic leukemia; Acute monoblastic/monocytic leukemia IC50=25 µM Resazurin assay 4h 1
U-937 Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia IC50=6.25 µM Resazurin assay 4h 1

Hemolytic Activity  Human erythrocytes: nonhemolytic up to 400 μM, and their equimolar mixture at that concentration caused only 7% hemolysis

Normal (non-cancerous) Cytotoxicity  Human Splenic Fibroblasts (HSF): IC50=12.78±0.33 µg/mL; Endometrial epithelial cells(HEEC): IC50=2.66±0.34 µg/mL; HPDE6c7 Human Pancreatic Duct Epithelial Cell: IC50=3.45±0.20 µg/mL; Normal Human Hepatic Cell (Lo-2): IC50=2.91±0.07 µg/mL; NCM460: IC50=3.04±0.10 µg/mL; CCD-19Lu: IC50=4.76±0.03 µg/mL

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00581

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C88H148N24O16

Absent amino acids  ACDEGHILMNPQTWY

Theoretical pI  11.47

Acidic residues  0

Basic residues  8

Polar residues  1

Molecular weight (Average)  1798.29

Molecular weight (Monoisotopic)  1797.15

Common amino acids  K

Net charge  8

Instability index (II)  2.98

Aliphatic index  41.43

Grand average of hydropathicity (GRAVY)  -1.129

Half Life 
  1.3 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  3 min (Escherichia coli, in vivo).

Extinction coefficients 
  Should not be visible by UV spectrophotometry.

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 26465972

Title  Structural Dissection of Crotalicidin, a Rattlesnake Venom Cathelicidin, Retrieves a Fragment with Antimicrobial and Antitumor Activity

Doi 10.1021/acs.jmedchem.5b01142

Year  2015

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.