This work is licensed under a Creative Commons Attribution 4.0 International License.
Ctn[1-14]
General Information
DCTPep ID DCTPep00581
Peptide Name Ctn[1-14]
Sequence KRFKKFFKKVKKSV
Sequence Length 14
UniProt ID Not available
PubChem CID Not available
Origin Rattlesnake Venom Cathelicidin
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa S3 | Breast cancer | IC50=1 µM | Resazurin assay | 4h | 1 |
| THP-1 | Childhood acute monocytic leukemia; Acute monoblastic/monocytic leukemia | IC50=25 µM | Resazurin assay | 4h | 1 |
| U-937 | Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia | IC50=6.25 µM | Resazurin assay | 4h | 1 |
Hemolytic Activity Human erythrocytes: nonhemolytic up to 400 μM, and their equimolar mixture at that concentration caused only 7% hemolysis
Normal (non-cancerous) Cytotoxicity Human Splenic Fibroblasts (HSF): IC50=12.78±0.33 µg/mL; Endometrial epithelial cells(HEEC): IC50=2.66±0.34 µg/mL; HPDE6c7 Human Pancreatic Duct Epithelial Cell: IC50=3.45±0.20 µg/mL; Normal Human Hepatic Cell (Lo-2): IC50=2.91±0.07 µg/mL; NCM460: IC50=3.04±0.10 µg/mL; CCD-19Lu: IC50=4.76±0.03 µg/mL
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00581
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C88H148N24O16
Absent amino acids ACDEGHILMNPQTWY
Theoretical pI 11.47
Acidic residues 0
Basic residues 8
Polar residues 1
Molecular weight (Average) 1798.29
Molecular weight (Monoisotopic) 1797.15
Common amino acids K
Net charge 8
Instability index (II) 2.98
Aliphatic index 41.43
Grand average of hydropathicity (GRAVY) -1.129
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 26465972
Title Structural Dissection of Crotalicidin, a Rattlesnake Venom Cathelicidin, Retrieves a Fragment with Antimicrobial and Antitumor Activity
Doi 10.1021/acs.jmedchem.5b01142
Year 2015
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.