Peptide Libraray

Temporin-1Sa [L2,9A;V6A]

General Information

DCTPep ID DCTPep00671

Peptide Name Temporin-1Sa [L2,9A;V6A]

Sequence FASGIAGMAGKLF

Sequence Length 13

UniProt ID Not available

PubChem CID Not available

Origin Synthetic

Type Synthetic peptide

Classification

Cancer therapy related peptides

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
THP-1	Childhood acute monocytic leukemia; Acute monoblastic/monocytic leukemia	IC50>60µM	MTT assay	72h	1
HepG2	Hepatoblastoma	IC50>600µM	MTT assay	72h	1

Hemolytic Activity Rat erythrocytes: 50% Hemolysis>200µM; Human erythrocytes: 50% Hemolysis >100µM

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00671

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C59H92N14O15S1

Absent amino acids CDEHNPQRTVWY

Theoretical pI 8.75

Acidic residues 0

Basic residues 1

Polar residues 4

Molecular weight (Average) 1269.52

Molecular weight (Monoisotopic) 1268.66

Common amino acids AG

Net charge 1

Instability index (II) -0.87

Aliphatic index 83.08

Grand average of hydropathicity (GRAVY) 1.177

Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).

Extinction coefficients
Should not be visible by UV spectrophotometry.

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 28319176

Title Insight into the mechanism of action of temporin-SHa, a new broad-spectrum antiparasitic and antibacterial agent

Doi 10.1371/journal.pone.0174024

Year 2017

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available