This work is licensed under a Creative Commons Attribution 4.0 International License.
BLP-7
General Information
DCTPep ID DCTPep00723
Peptide Name BLP-7
Sequence GIGGALLSAGKSALKGLAKGLAEHFAN
Sequence Length 27
UniProt ID Q9DET7 Q58T59 Q58T89 A0A219CM62
PubChem CID Not available
Origin skin secretion of Oriental fire-bellied toad, Bombina orientalis
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| SK-HEP-1 | Liver and intrahepatic bile duct epithelial neoplasm | IC50=0.61 μM | MTT assay | 48h | 1 |
| Hep-G2 | Hepatoblastoma; Hepatoblastoma | IC50=2.83 μM | MTT assay | 48h | 1 |
| Huh-7 | Adult hepatocellular carcinoma; Adult hepatocellular carcinoma | IC50=3.87 μM | MTT assay | 48h | 1 |
Hemolytic Activity Human erythrocytes: BLP-7 exhibited rather low cytotoxicity on human erythrocytes at concentration of 50.2 μm (128 mg/ml), and almost no haemolytic activity was detected around its range of MIC values.
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00723
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C114H191N33O33
Absent amino acids CDMPQRTVWY
Theoretical pI 9.70
Acidic residues 1
Basic residues 4
Polar residues 9
Molecular weight (Average) 2551.97
Molecular weight (Monoisotopic) 2550.43
Common amino acids AG
Net charge 3
Instability index (II) -11.3
Aliphatic index 108.89
Grand average of hydropathicity (GRAVY) 0.415
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28636781
Title Discovery of two bombinin peptides with antimicrobial and anticancer activities from the skin secretion of Oriental fire-bellied toad, Bombina orientalis
Year 2018
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.