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Bombinin H-BO
General Information
DCTPep ID DCTPep00724
Peptide Name Bombinin H-BO
Sequence IIGPVLGLIGKALGGLL
Sequence Length 17
UniProt ID Not available
PubChem CID Not available
Origin skin secretion of Oriental fire-bellied toad, Bombina orientalis
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| SK-HEP-1 | Liver and intrahepatic bile duct epithelial neoplasm | IC50=0.99 μM | MTT assay | 48h | 1 |
| Huh-7 | Adult hepatocellular carcinoma; Adult hepatocellular carcinoma | IC50=1.81 μM | MTT assay | 48h | 1 |
| Hep-G2 | Hepatoblastoma; Hepatoblastoma | IC50=2.88 μM | MTT assay | 48h | 1 |
Hemolytic Activity Human erythrocytes: Bombinin H-BO was found to lyse 38% of erythrocytes at concentration of 80.0 μm (128 mg/ml), and up to 85% at the concentration of 159.7 μm (256 mg/ml).
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00724
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C77H138N18O18
Absent amino acids CDEFHMNQRSTWY
Theoretical pI 8.75
Acidic residues 0
Basic residues 1
Polar residues 5
Molecular weight (Average) 1604.05
Molecular weight (Monoisotopic) 1603.04
Common amino acids GL
Net charge 1
Instability index (II) 23.01
Aliphatic index 206.47
Grand average of hydropathicity (GRAVY) 1.824
Half Life
20 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28636781
Title Discovery of two bombinin peptides with antimicrobial and anticancer activities from the skin secretion of Oriental fire-bellied toad, Bombina orientalis
Year 2018
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.