This work is licensed under a Creative Commons Attribution 4.0 International License.
Phylloseptin-PHa
General Information
DCTPep ID DCTPep00776
Peptide Name Phylloseptin-PHa
Sequence FLSLIPAAISAVSALANHF
Sequence Length 19
UniProt ID Not available
PubChem CID Not available
Origin Pithecopus hypochondrialis
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MCF-7 | Invasive breast carcinoma of no special type | LD50=24.05µM | MTT assay | 24h | 1 |
| MDA-MB-435S | Amelanotic melanoma | LD50=28.48µM | MTT assay | 24h | 1 |
| U-251MG | Astrocytoma | LD50=34.67µM | MTT assay | 24h | 1 |
| HCT 116 | Colon carcinoma | LD50=36.64µM | MTT assay | 24h | 1 |
| NCI-H157 | Lung squamous cell carcinoma | LD50=37.61µM | MTT assay | 24h | 1 |
| PC-3 | Prostate carcinoma | LD50=37.96µM | MTT assay | 24h | 1 |
Hemolytic Activity Horse erythrocytes: 10% Hemolysis=76.5µM; 50% Hemolysis=109.5µM; 50% Hemolysis=52.17µM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00776
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C92H144N22O24
Absent amino acids CDEGKMQRTWY
Theoretical pI 6.74
Acidic residues 0
Basic residues 1
Polar residues 4
Molecular weight (Average) 1942.29
Molecular weight (Monoisotopic) 1941.07
Common amino acids A
Net charge 1
Instability index (II) 12.64
Aliphatic index 144.21
Grand average of hydropathicity (GRAVY) 1.500
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28850103
Title Discovery of Phylloseptins that Defense against Gram-Positive Bacteria and Inhibit the Proliferation of the Non-Small Cell Lung Cancer Cell Line, from the Skin Secretions of Phyllomedusa Frogs
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.