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HlDFS1
General Information
DCTPep ID DCTPep00798
Peptide Name HlDFS1
Sequence GFGCPFNARRCHRHCRSIRRRAGYCAGRLRLTCTCVR
Sequence Length 37
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
Cancer therapy related peptides
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| A549 | Lung adenocarcinoma | Not active up to 20 µM | Cell Viability Assay | 24h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL29 positive; Chronic myeloid leukemia | Not active up to 20 µM | Cell Viability Assay | 24h | 1 |
| THP-1 | Childhood acute monocytic leukemia; Acute monoblastic/monocytic leukemia | Not active up to 20 µM | Cell Viability Assay | 24h | 1 |
Hemolytic Activity Human erythrocytes: <5% Hemolysis=10µM; 18% Hemolysis=20µM; 60% Hemolysis=50µM
Normal (non-cancerous) Cytotoxicity HEK293T: Not active up to 20 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00798
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys4<--->Cys25; Cys11<--->Cys33; Cys15<--->Cys35
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C177H294N72O43S6
Absent amino acids DEKMQW
Theoretical pI 11.41
Acidic residues 0
Basic residues 12
Polar residues 15
Molecular weight (Average) 4311.1
Molecular weight (Monoisotopic) 4308.14
Common amino acids R
Net charge 12
Instability index (II) 102.16
Aliphatic index 47.57
Grand average of hydropathicity (GRAVY) -0.522
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 1865
Abs 0.1% (=1 g/l) 0.433, assuming all pairs of Cys residues form cystines
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 0.346, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28969703
Title Functional characterization of two defensins, HlDFS1 and HlDFS2, from the hard tick Haemaphysalis longicornis
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.