HlDFS2

General Information


DCTPep ID  DCTPep00799

Peptide Name   HlDFS2

Sequence  GFGCPLNQGACHRHCRSIRRRGGYCSGIIKQTCTCY

Sequence Length  36

UniProt ID  Not available

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

Cancer therapy related peptides



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
A549 Lung adenocarcinoma Not active up to 20 µM Cell Viability Assay 24h 1
K562 Blast phase chronic myelogenous leukemia, BCR-ABL30 positive; Chronic myeloid leukemia Not active up to 20 µM Cell Viability Assay 24h 1
THP-1 Childhood acute monocytic leukemia; Acute monoblastic/monocytic leukemia Not active up to 20 µM Cell Viability Assay 24h 1

Hemolytic Activity  Human erythrocytes: <5% Hemolysis=50µM

Normal (non-cancerous) Cytotoxicity  HEK293T: Not active up to 20 µM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00799

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys4<--->Cys25; Cys11<--->Cys33; Cys15<--->Cys35

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C165H265N59O46S6

Absent amino acids  DEMVW

Theoretical pI  9.43

Acidic residues  0

Basic residues  8

Polar residues  19

Molecular weight (Average)  4003.65

Molecular weight (Monoisotopic)  4000.85

Common amino acids  CG

Net charge  8

Instability index (II)  90.89

Aliphatic index  46.11

Grand average of hydropathicity (GRAVY)  -0.444

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 3355
  Abs 0.1% (=1 g/l) 0.838, assuming all pairs of Cys residues form cystines
  Ext. coefficient 2980
  Abs 0.1% (=1 g/l) 0.744, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 28969703

Title  Functional characterization of two defensins, HlDFS1 and HlDFS2, from the hard tick Haemaphysalis longicornis

Doi 10.1186/s13071-017-2397-9

Year  2017

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.