This work is licensed under a Creative Commons Attribution 4.0 International License.
Dermaseptin-PH
General Information
DCTPep ID DCTPep00800
Peptide Name Dermaseptin-PH
Sequence ALWKEVLKNAGKAALNEINNLVQ
Sequence Length 23
UniProt ID Not available
PubChem CID Not available
Origin Phyllomedusa hypochondrialis
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MCF-7 | Invasive breast carcinoma of no special type | IC50=0.69µM | MTT assay | 24h | 1 |
| PC-3 | Prostate carcinoma | IC50=11.8µM | MTT assay | 24h | 1 |
| NCI-H157 | Lung squamous cell carcinoma | IC50=2.01µM | MTT assay | 24h | 1 |
| U-251MG | Astrocytoma | IC50=2.36µM | MTT assay | 24h | 1 |
| MDA-MB-435S | Amelanotic melanoma | IC50=9.94µM | MTT assay | 24h | 1 |
Hemolytic Activity Horse erythrocytes: 1% Hemolysis=16µM; 3% Hemolysis=64µM; 10% Hemolysis=128µM; 45% Hemolysis=256µM; 100% Hemolysis=512µM
Normal (non-cancerous) Cytotoxicity HMEC-1: IC50=4.85µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00800
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C114H190N32O33
Absent amino acids CDFHMPRSTY
Theoretical pI 8.54
Acidic residues 2
Basic residues 3
Polar residues 5
Molecular weight (Average) 2536.96
Molecular weight (Monoisotopic) 2535.42
Common amino acids ALN
Net charge 1
Instability index (II) 20.85
Aliphatic index 127.39
Grand average of hydropathicity (GRAVY) -0.096
Half Life
4.4 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.168
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 29064402
Title Dermaseptin-PH: A Novel Peptide with Antimicrobial and Anticancer Activities from the Skin Secretion of the South American Orange-Legged Leaf Frog, Pithecopus (Phyllomedusa) hypochondrialis
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.