This work is licensed under a Creative Commons Attribution 4.0 International License.
Bombinin-BO1
General Information
DCTPep ID DCTPep00802
Peptide Name Bombinin-BO1
Sequence GIGSAILSAGKSIIKGLAKGLA
Sequence Length 22
UniProt ID A0A219CMY0
PubChem CID Not available
Origin skin secretion of Oriental fire-bellied toad, Bombina orientalis
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| Hep-G2 | Hepatoblastoma; Hepatoblastoma | IC50=0.76 μM | MTT assay | 48h | 1 |
| SK-HEP-1 | Liver and intrahepatic bile duct epithelial neoplasm | IC50=3.75 μM | MTT assay | 48h | 1 |
| Huh-7 | Adult hepatocellular carcinoma; Adult hepatocellular carcinoma | IC50=3.91 μM | MTT assay | 48h | 1 |
Hemolytic Activity Human Red cells: only 2.89% hemolysis of the red cells at a concentration of 26.3 μM and 38.05% hemolysis at 52.5 μM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00802
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C91H165N25O26
Absent amino acids CDEFHMNPQRTVWY
Theoretical pI 10.30
Acidic residues 0
Basic residues 3
Polar residues 8
Molecular weight (Average) 2025.46
Molecular weight (Monoisotopic) 2024.24
Common amino acids G
Net charge 3
Instability index (II) -1
Aliphatic index 142.27
Grand average of hydropathicity (GRAVY) 0.932
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 29098406
Title Molecular characterization and bioactivity evaluation of two novel bombinin peptides from the skin secretion of Oriental fire-bellied toad, Bombina orientalis
Year 2018
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.