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Bombinin H-BO1
General Information
DCTPep ID DCTPep00803
Peptide Name Bombinin H-BO1
Sequence IIGPVLGLVGKALGGLL
Sequence Length 17
UniProt ID Not available
PubChem CID Not available
Origin skin secretion of Oriental fire-bellied toad, Bombina orientalis
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| Hep-G2 | Hepatoblastoma; Hepatoblastoma | IC50=3.61 μM | MTT assay | 48h | 1 |
| SK-HEP-1 | Liver and intrahepatic bile duct epithelial neoplasm | IC50=8.08 μM | MTT assay | 48h | 1 |
| Huh-7 | Adult hepatocellular carcinoma; Adult hepatocellular carcinoma | IC50=8.42 μM | MTT assay | 48h | 1 |
Hemolytic Activity Human Red cells: caused 42.03% hemolysis of the red cells at a concentration of 40.3 μM while reaching 100% hemolysis at 161.1 μM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00803
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C76H136N18O18
Absent amino acids CDEFHMNQRSTWY
Theoretical pI 8.75
Acidic residues 0
Basic residues 1
Polar residues 5
Molecular weight (Average) 1590.03
Molecular weight (Monoisotopic) 1589.03
Common amino acids GL
Net charge 1
Instability index (II) 18.01
Aliphatic index 200.59
Grand average of hydropathicity (GRAVY) 1.806
Half Life
20 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 29098406
Title Molecular characterization and bioactivity evaluation of two novel bombinin peptides from the skin secretion of Oriental fire-bellied toad, Bombina orientalis
Year 2018
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.