This work is licensed under a Creative Commons Attribution 4.0 International License.
Temporin-PE
General Information
DCTPep ID DCTPep00806
Peptide Name Temporin-PE
Sequence FLPIVAKLLSGLL
Sequence Length 13
UniProt ID A0A2H4WAJ8
PubChem CID Not available
Origin Pelophylax kl.esculentus(Europe edible frog)
Type Native peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| U-251MG | Astrocytoma | IC50=25.13µM | MTT assay | Not available | 1 |
| MDA-MB-435s | Amelanotic melanoma | IC50=33.23µM | MTT assay | Not available | 1 |
| NCI-H157 | Lung squamous cell carcinoma | IC50=34.56µM | MTT assay | Not available | 1 |
| PC-3 | Prostate carcinoma | IC50=38.56µM | MTT assay | Not available | 1 |
Hemolytic Activity Horse erythrocytes(HD50=39.64μM)
Normal (non-cancerous) Cytotoxicity HMEC-1: IC50=58.62µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00806
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Not available
C-terminal Modification Not available
Other Modification None
Chiral L
Physicochemical Information
Formula C69H118N14O15
Absent amino acids CDEHMNQRTWY
Theoretical pI 8.75
Acidic residues 0
Basic residues 1
Polar residues 2
Molecular weight (Average) 1383.78
Molecular weight (Monoisotopic) 1382.89
Common amino acids L
Net charge 1
Instability index (II) 10.98
Aliphatic index 210.00
Grand average of hydropathicity (GRAVY) 1.969
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 29191658
Title Identification and target-modifications of temporin-PE: A novel antimicrobial peptide in the defensive skin secretions of the edible frog, Pelophylax kl. Esculentus
Doi 10.1016/j.bbrc.2017.11.173
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.