Peptide Libraray

Temporin-PE - Tat (48–57)

General Information

DCTPep ID DCTPep00807

Peptide Name Temporin-PE - Tat (48–57)

Sequence FLPIVAKLLSGLLGRKKRRQRRR

Sequence Length 23

UniProt ID Not available

PubChem CID Not available

Origin Synthetic

Type Synthetic peptide

Classification

ACP Tumor active peptide Membrane-targeted

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
PC-3	Prostate carcinoma	IC50=3.051µM	MTT assay	24h	1
U-251MG	Astrocytoma	IC50=3.087µM	MTT assay	24h	1
NCI-H157	Lung squamous cell carcinoma	IC50=3.464µM	MTT assay	24h	1
MDA-MB-435S	Amelanotic melanoma	IC50=3.483µM	MTT assay	24h	1

Hemolytic Activity Horse erythrocytes: 50% Hemolysis=44.65µM

Normal (non-cancerous) Cytotoxicity HMEC-1: IC50=9.976µM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00807

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C124H225N45O26

Absent amino acids CDEHMNTWY

Theoretical pI 12.70

Acidic residues 0

Basic residues 9

Polar residues 3

Molecular weight (Average) 2762.44

Molecular weight (Monoisotopic) 2760.77

Common amino acids R

Net charge 9

Instability index (II) 107.82

Aliphatic index 118.70

Grand average of hydropathicity (GRAVY) -0.570

Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).

Extinction coefficients
Should not be visible by UV spectrophotometry.

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 29191658

Title Identification and target-modifications of temporin-PE: A novel antimicrobial peptide in the defensive skin secretions of the edible frog, Pelophylax kl. Esculentus

Doi 10.1016/j.bbrc.2017.11.173

Year 2018

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available