This work is licensed under a Creative Commons Attribution 4.0 International License.
Temporin-PE [P3Y]
General Information
DCTPep ID DCTPep00808
Peptide Name Temporin-PE [P3Y]
Sequence FLYIVAKLLSGLL
Sequence Length 13
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| PC-3 | Prostate carcinoma | IC50=27.62µM | MTT assay | 24h | 1 |
| NCI-H157 | Lung squamous cell carcinoma | IC50=3.398µM | MTT assay | 24h | 1 |
| U-251MG | Astrocytoma | IC50=3.520µM | MTT assay | 24h | 1 |
| MDA-MB-435S | Amelanotic melanoma | IC50=9.864µM | MTT assay | 24h | 1 |
Hemolytic Activity Horse erythrocytes: 50% Hemolysis=526.5µM
Normal (non-cancerous) Cytotoxicity HMEC-1: IC50=40.30µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00808
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C73H120N14O16
Absent amino acids CDEHMNPQRTW
Theoretical pI 8.59
Acidic residues 0
Basic residues 1
Polar residues 3
Molecular weight (Average) 1449.84
Molecular weight (Monoisotopic) 1448.9
Common amino acids L
Net charge 1
Instability index (II) -3.83
Aliphatic index 210.00
Grand average of hydropathicity (GRAVY) 1.992
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 1.028
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 29191658
Title Identification and target-modifications of temporin-PE: A novel antimicrobial peptide in the defensive skin secretions of the edible frog, Pelophylax kl. Esculentus
Doi 10.1016/j.bbrc.2017.11.173
Year 2018
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.