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TI
General Information
DCTPep ID DCTPep00916
Peptide Name TI
Sequence KWCFRVCYRGICYRRCR
Sequence Length 17
PubChem CID 16129721
Origin host defense peptide from the horseshoe crab Tachypleus tridentatus
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MM96L | Melanoma | CC50=1.5±0.1 µM | Resazurin assay | 24h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | CC50=1.7±0.1 µM | Resazurin assay | 24h | 1 |
| HT-144 | Melanoma | CC50=1.7±0.2 µM | Resazurin assay | 24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | CC50=13.1±1.2 µM | Resazurin assay | 24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | CC50=16.0±2.0 µM | Resazurin assay | 24h | 1 |
| WM164 | Cutaneous melanoma | CC50=2.5±0.1 µM | Resazurin assay | 24h | 1 |
| MDA-MB-435S | Amelanotic melanoma | CC50=8.0±0.6 µM | Resazurin assay | 24h | 1 |
Hemolytic Activity Human RBCs: CC50=34.9±2.8 µM
Normal (non-cancerous) Cytotoxicity HaCaT: CC50=16.0±1.5 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
Predicted Structure DCTPep00916
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys3<--->Cys16; Cys7<--->Cys12
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C99H154N34O20S4
Absent amino acids ADEHLMNPQST
Theoretical pI 9.93
Acidic residues 0
Basic residues 6
Polar residues 7
Molecular weight (Average) 2268.77
Molecular weight (Monoisotopic) 2267.1
Common amino acids R
Net charge 6
Instability index (II) 13.22
Aliphatic index 40.00
Grand average of hydropathicity (GRAVY) -0.518
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 8730
Abs 0.1% (=1 g/l) 3.848, assuming all pairs of Cys residues form cystines
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 3.738, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 31714739
Title Cyclic Analogues of Horseshoe Crab Peptide Tachyplesin I with Anticancer and Cell Penetrating Properties
Doi 10.1021/acschembio.9b00782
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.