This work is licensed under a Creative Commons Attribution 4.0 International License.
[F4S-Y13S]cTI
General Information
DCTPep ID DCTPep00923
Peptide Name [F4S-Y13S]cTI
Sequence KWCSRVCYRGICSRRCRG
Sequence Length 18
UniProt ID Not available
PubChem CID Not available
Origin host defense peptide from the horseshoe crab Tachypleus tridentatus
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | CC50=13.2±0.9 µM | Resazurin assay | 24h | 1 |
| MM96L | Melanoma | CC50=17.3±1.0 µM | Resazurin assay | 24h | 1 |
| HT-144 | Melanoma | CC50=27.8±2.2 µM | Resazurin assay | 24h | 1 |
| WM164 | Cutaneous melanoma | CC50=28.2±2.0 µM | Resazurin assay | 24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | CC50>32 µM | Resazurin assay | 24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | CC50>32 µM | Resazurin assay | 24h | 1 |
| MDA-MB-435S | Amelanotic melanoma | CC50>64 µM | Resazurin assay | 24h | 1 |
Hemolytic Activity Human RBCs: CC50>64 µM
Normal (non-cancerous) Cytotoxicity HaCaT: CC50>64 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00923
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys3<--->Cys16; Cys7<--->Cys12
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C89H149N35O22S4
Absent amino acids ADEFHLMNPQT
Theoretical pI 10.16
Acidic residues 0
Basic residues 6
Polar residues 9
Molecular weight (Average) 2189.62
Molecular weight (Monoisotopic) 2188.05
Common amino acids R
Net charge 6
Instability index (II) 39.12
Aliphatic index 37.78
Grand average of hydropathicity (GRAVY) -0.683
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 7240
Abs 0.1% (=1 g/l) 3.307, assuming all pairs of Cys residues form cystines
Ext. coefficient 6990
Abs 0.1% (=1 g/l) 3.192, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 31714739
Title Cyclic Analogues of Horseshoe Crab Peptide Tachyplesin I with Anticancer and Cell Penetrating Properties
Doi 10.1021/acschembio.9b00782
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.