Brevinin-1H

General Information


DCTPep ID  DCTPep01011

Peptide Name   Brevinin-1H

Sequence  FALGAVTKVLPKLFCLITRKC

Sequence Length  21

UniProt ID  Not available

PubChem CID  Not available

Origin  skin secretion of Amolops hainanensis 

Type  Native peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HCT 116 Colon carcinoma IC50=2.599 μM MTT assay 24h 1
NCI-H157 Lung squamous cell carcinoma IC50=3.37 μM MTT assay 24h 1
MDA-MB-435S Amelanotic melanoma IC50=3.47 μM MTT assay 24h 1
PC-3 Prostate carcinoma IC50=5.87 μM MTT assay 24h 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Horse blood cells: HC50=53.12 µM

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep01011

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C109H185N27O24S2

Absent amino acids  DEHMNQSWY

Theoretical pI  9.85

Acidic residues  0

Basic residues  4

Polar residues  5

Molecular weight (Average)  2321.95

Molecular weight (Monoisotopic)  2320.35

Common amino acids  L

Net charge  4

Instability index (II)  11.7

Aliphatic index  130.00

Grand average of hydropathicity (GRAVY)  1.081

Half Life 
  1.1 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 125
  Abs 0.1% (=1 g/l) 0.054, assuming all pairs of Cys residues form cystines
  Ext. coefficient 0
  Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 32812694

Title  Characterisation of a novel peptide, Brevinin-1H, from the skin secretion of Amolops hainanensis and rational design of several analogues

Doi 10.1111/cbdd.13779

Year  2021

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.