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Brevinin-1Ha
General Information
DCTPep ID DCTPep01012
Peptide Name Brevinin-1Ha
Sequence FALGAVTCLIRTKCKVLPKLF
Sequence Length 21
UniProt ID Not available
PubChem CID Not available
Origin skin secretion of Amolops hainanensis
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| PC-3 | Prostate carcinoma | IC50=186.9 μM | MTT assay | 24h | 1 |
| MDA-MB-435S | Amelanotic melanoma | IC50=214.7 μM | MTT assay | 24h | 1 |
| NCI-H157 | Lung squamous cell carcinoma | IC50=278.9 μM | MTT assay | 24h | 1 |
| HCT 116 | Colon carcinoma | IC50=342.8 μM | MTT assay | 24h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity Horse blood cells: only over 20% at a concentration of 128 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep01012
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C109H185N27O24S2
Absent amino acids DEHMNQSWY
Theoretical pI 9.85
Acidic residues 0
Basic residues 4
Polar residues 5
Molecular weight (Average) 2321.95
Molecular weight (Monoisotopic) 2320.35
Common amino acids L
Net charge 4
Instability index (II) 11.7
Aliphatic index 130.00
Grand average of hydropathicity (GRAVY) 1.081
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.054, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 32812694
Title Characterisation of a novel peptide, Brevinin-1H, from the skin secretion of Amolops hainanensis and rational design of several analogues
Year 2021
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID 16101
This work is licensed under a Creative Commons Attribution 4.0 International License.