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Brevinin-1HY
General Information
DCTPep ID DCTPep01013
Peptide Name Brevinin-1HY
Sequence FALGAVTKVLYKLFCLITRKC
Sequence Length 21
UniProt ID Not available
PubChem CID Not available
Origin skin secretion of Amolops hainanensis
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MDA-MB-435S | Amelanotic melanoma | IC50=2.243 μM | MTT assay | 24h | 1 |
| NCI-H157 | Lung squamous cell carcinoma | IC50=2.811 μM | MTT assay | 24h | 1 |
| HCT 116 | Colon carcinoma | IC50=3.583 μM | MTT assay | 24h | 1 |
| PC-3 | Prostate carcinoma | IC50=5.425 μM | MTT assay | 24h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity Horse blood cells: The haemolysis at the MIC of Brevinin-1HY (28.48%) was more than threefold at the MIC for the parent peptide
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep01013
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C113H187N27O25S2
Absent amino acids DEHMNPQSW
Theoretical pI 9.70
Acidic residues 0
Basic residues 4
Polar residues 6
Molecular weight (Average) 2388.01
Molecular weight (Monoisotopic) 2386.36
Common amino acids L
Net charge 4
Instability index (II) 2.52
Aliphatic index 130.00
Grand average of hydropathicity (GRAVY) 1.095
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 1615
Abs 0.1% (=1 g/l) 0.676, assuming all pairs of Cys residues form cystines
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 0.624, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 32812694
Title Characterisation of a novel peptide, Brevinin-1H, from the skin secretion of Amolops hainanensis and rational design of several analogues
Year 2021
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.