Peptide Libraray

Temporin-SHe

General Information

DCTPep ID DCTPep01019

Peptide Name Temporin-SHe

Sequence FLPALAGIAGLLGKIF

Sequence Length 16

UniProt ID Not available

PubChem CID Not available

Origin Pelophylax saharicus

Type Native peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
THP-1	Childhood acute monocytic leukemia; Acute monoblastic/monocytic leukemia	45% Killing=12.5µM; 96% Killing=25µM	MTT assay	72h	1

Hemolytic Activity Human erythrocytes: 22% Hemolysis=12.5µM; 84% Hemolysis=25µM

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep01019

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C80H129N17O17

Absent amino acids CDEHMNQRSTVWY

Theoretical pI 8.75

Acidic residues 0

Basic residues 1

Polar residues 3

Molecular weight (Average) 1601.01

Molecular weight (Monoisotopic) 1599.98

Common amino acids L

Net charge 1

Instability index (II) 17.53

Aliphatic index 165.00

Grand average of hydropathicity (GRAVY) 1.781

Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).

Extinction coefficients
Should not be visible by UV spectrophotometry.

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 32933215

Title Functional Characterization of Temporin-SHe, a New Broad-Spectrum Antibacterial and Leishmanicidal Temporin-SH Paralog from the Sahara Frog ( Pelophylax saharicus)

Doi 10.3390/ijms21186713

Year 2020

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available