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MAD1
General Information
DCTPep ID DCTPep01035
Peptide Name MAD1
Sequence KRWHWWRRHWVVW
Sequence Length 13
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| OVCAR-3 | High grade ovarian serous adenocarcinoma | IC50=14.2 µM | MTT assay | 48h | 1 |
| A549 | Lung adenocarcinoma | IC50=36.2 µM | MTT assay | 48h | 1 |
| NCI/ADR-RES | High grade ovarian serous adenocarcinoma | IC50=85.6 µM | MTT assay | 48h | 1 |
| T24 | Bladder carcinoma | IC50>100 µM | MTT assay | 48h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity HUVEC: IC50>100 μM; NL20: IC50=76.9 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep01035
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C101H132N32O14
Absent amino acids ACDEFGILMNPQSTY
Theoretical pI 12.30
Acidic residues 0
Basic residues 6
Polar residues 0
Molecular weight (Average) 2018.37
Molecular weight (Monoisotopic) 2017.06
Common amino acids W
Net charge 6
Instability index (II) 144.5
Aliphatic index 44.62
Grand average of hydropathicity (GRAVY) -1.531
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 27500
Abs 0.1% (=1 g/l) 13.625
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 33184577
Title Re-engineering Antimicrobial Peptides into Oncolytics Targeting Drug-Resistant Ovarian Cancers
Doi 10.1007/s12195-020-00626-z
Year 2020
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.