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Brevilaterin B
General Information
DCTPep ID DCTPep01102
Peptide Name Brevilaterin B
Sequence XXMXIVVKVLKYLX
Sequence Length 14
UniProt ID Not available
PubChem CID Not available
Origin Brevibacillus laterosporus
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HT-29 | Colon adenocarcinoma | IC50=1.40±0.09 µg/mL | CCK-8 assay | 48h | 1 |
| SW1990 | Pancreatic adenocarcinoma | IC50=13.40±0.08 µg/mL | CCK-8 assay | 48h | 1 |
| SMMC-7721 | Human papillomavirus-related endocervical adenocarcinoma | IC50=13.81±0.31 µg/mL | CCK-8 assay | 48h | 1 |
| SW480 | Colon adenocarcinoma | IC50=17.15±0.97 µg/mL | CCK-8 assay | 48h | 1 |
| EC109 | Esophageal cancer | IC50=2.13±0.23 µg/mL | CCK-8 assay | 48h | 1 |
| A498 | Renal cell carcinoma | IC50=2.33±0.49 µg/mL | CCK-8 assay | 48h | 1 |
| DU145 | Prostate carcinoma | IC50=2.56±0.23 µg/mL | CCK-8 assay | 48h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50=2.60±0.24 µg/mL | CCK-8 assay | 48h | 1 |
| A431 | Skin squamous cell carcinoma | IC50=2.75±0.07 µg/mL | CCK-8 assay | 48h | 1 |
| PANC-1 | Pancreatic ductal adenocarcinoma | IC50=3.11±0.49 µg/mL | CCK-8 assay | 48h | 1 |
| KB | Human papillomavirus-related endocervical adenocarcinoma | IC50=3.14±0.07 µg/mL | CCK-8 assay | 48h | 1 |
| HeLa S3 | Breast cancer | IC50=3.16±0.45 µg/mL | CCK-8 assay | 48h | 1 |
| EC9706 | Esophageal squamous cell carcinoma; Squamous cell carcinoma of the esophagus | IC50=3.23±0.16 µg/mL | CCK-8 assay | 48h | 1 |
| A-375 | Amelanotic melanoma | IC50=4.16±0.15 µg/mL | CCK-8 assay | 48h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | IC50=4.16±0.27 µg/mL | CCK-8 assay | 48h | 1 |
| SK-OV-3 | Ovarian serous cystadenocarcinoma | IC50=4.26±0.25 µg/mL | CCK-8 assay | 48h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=4.94±0.28 µg/mL | CCK-8 assay | 48h | 1 |
| A549 | Lung adenocarcinoma | IC50=5.54±0.55 µg/mL | CCK-8 assay | 48h | 1 |
| BGC-823 | Human papillomavirus-related endocervical adenocarcinoma | IC50=5.68±0.25 µg/mL | CCK-8 assay | 48h | 1 |
| SGC-7901 | Human papillomavirus-related endocervical adenocarcinoma | IC50=5.73±0.21 µg/mL | CCK-8 assay | 48h | 1 |
| Tca8113 | Human papillomavirus-related endocervical adenocarcinoma | IC50=5.82±0.25 µg/mL | CCK-8 assay | 48h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | IC50=5.86±0.66 µg/mL | CCK-8 assay | 48h | 1 |
| PC-3M | Prostate carcinoma | IC50=6.17±0.24 µg/mL | CCK-8 assay | 48h | 1 |
| A2780 | Ovarian endometrioid adenocarcinoma; Endometrioid carcinoma of ovary | IC50=7.40±0.36 µg/mL | CCK-8 assay | 48h | 1 |
| HCT 116 | Colon carcinoma | IC50=7.67±0.57 µg/mL | CCK-8 assay | 48h | 1 |
| Hep-G2 | Hepatoblastoma; Hepatoblastoma | IC50=9.84±0.39 µg/mL | CCK-8 assay | 48h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity Human Splenic Fibroblasts (HSF): IC50=12.78±0.33 µg/mL; Endometrial epithelial cells(HEEC): IC50=2.66±0.34 µg/mL; HPDE6c7 Human Pancreatic Duct Epithelial Cell: IC50=3.45±0.20 µg/mL; Normal Human Hepatic Cell (Lo-2): IC50=2.91±0.07 µg/mL; NCM460: IC50=3.04±0.10 µg/mL; CCD-19Lu: IC50=4.76±0.03 µg/mL; Human Intrahepatic Biliary Epithelial Cells (HIBEpiC): IC50=2.37±0.38 µg/; HEK293: IC50=4.78±0.32 µg/mL; IOSE80: IC50=1.12±0.04 µg/mL; RWPE-1: IC50=1.07±0.34 µg/mL; GES-1: IC50=10.62±0.73 µg/mL; MCF-10A: IC50=1.58±0.33 µg/mL;
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure Not available
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification X(1)=Hmp=2-hydroxy-3-methylpentanoic acid; X(2)=Aba=2-amino-2-butenoic acid; X(4)=Ornithine; X(14)=valinol
Chiral L
Physicochemical Information
Formula Not Applicable
Absent amino acids Not Applicable
Theoretical pI Not Applicable
Acidic residues Not Applicable
Basic residues Not Applicable
Polar residues Not Applicable
Molecular weight (Average) Not Applicable
Molecular weight (Monoisotopic) Not Applicable
Common amino acids Not Applicable
Net charge Not Applicable
Instability index (II) Not Applicable
Aliphatic index Not Applicable
Grand average of hydropathicity (GRAVY) Not Applicable
Half Life
Not Applicable
Extinction coefficients
Not Applicable
Amino acid distribution
Literature Information
Literature 1
Pubmed ID Not available
Title Brevilaterin B from Brevibacillus laterosporus has selective antitumor activity and induces apoptosis in epidermal cancer
Doi 10.21203/rs.3.rs-955399/v1
Year 2021
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.