HYL-14

General Information


DCTPep ID  DCTPep01200

Peptide Name   HYL-14

Sequence  GIMSSLMKKLⓍAHIⓍK

Sequence Length  16

UniProt ID  Not available

PubChem CID  Not available

Origin  AMP

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Stapled Peptides



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
A549 Lung adenocarcinoma IC50=1.36±0.35 µmol/L MTT assay 72h Patent
HCT 116 Colon carcinoma IC50=1.61±0.27 µmol/L MTT assay 72h Patent
Hep-G2 Hepatoblastoma; Hepatoblastoma IC50=1.67±0.25 µmol/L MTT assay 72h Patent

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Human Splenic Fibroblasts (HSF): IC50=12.78±0.33 µg/mL; Endometrial epithelial cells(HEEC): IC50=2.66±0.34 µg/mL; HPDE6c7 Human Pancreatic Duct Epithelial Cell: IC50=3.45±0.20 µg/mL; Normal Human Hepatic Cell (Lo-2): IC50=2.91±0.07 µg/mL; NCM460: IC50=3.04±0.10 µg/mL; CCD-19Lu: IC50=4.76±0.03 µg/mL

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  Not available

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Cyclic (Stapled)

Disulfide/Other Bond  Stapled: Ⓧ(11) and Ⓧ (15) are cross-linked by hydrocarbon stapling

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  Ⓧ=S5=(S)-N-Fmoc-2-(4-pentenyl)alanine

Chiral  L



Physicochemical Information


Formula  Not Applicable

Absent amino acids  Not Applicable

Theoretical pI  Not Applicable

Acidic residues  Not Applicable

Basic residues  Not Applicable

Polar residues  Not Applicable

Molecular weight (Average)  Not Applicable

Molecular weight (Monoisotopic)  Not Applicable

Common amino acids  Not Applicable

Net charge  Not Applicable

Instability index (II)  Not Applicable

Aliphatic index  Not Applicable

Grand average of hydropathicity (GRAVY)  Not Applicable

Half Life 
  Not Applicable

Extinction coefficients 
  Not Applicable

Amino acid distribution



Literature Information


Literature 1

Pubmed ID Not available

Title  Structural modification and antitumor activity of antimicrobial peptide HYL

Doi 10.1016/j.cclet.2019.10.013

Year  2020

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.