HYL

General Information


DCTPep ID  DCTPep01204

Peptide Name   HYL

Sequence  GIMSSLMKKLAAHIAK

Sequence Length  16

UniProt ID  Not available

PubChem CID  Not available

Origin  AMP

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
A549 Lung adenocarcinoma IC50=10.01±0.34 µmol/L MTT assay 72h Patent
Hep-G2 Hepatoblastoma; Hepatoblastoma IC50=14.71±0.42 µmol/L MTT assay 72h Patent
HCT 116 Colon carcinoma IC50=22.89±0.22 µmol/L MTT assay 72h Patent

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Human Splenic Fibroblasts (HSF): IC50=12.78±0.33 µg/mL; Endometrial epithelial cells(HEEC): IC50=2.66±0.34 µg/mL; HPDE6c7 Human Pancreatic Duct Epithelial Cell: IC50=3.45±0.20 µg/mL; Normal Human Hepatic Cell (Lo-2): IC50=2.91±0.07 µg/mL; NCM460: IC50=3.04±0.10 µg/mL; CCD-19Lu: IC50=4.76±0.03 µg/mL

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep01204

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C75H135N21O19S2

Absent amino acids  CDEFNPQRTVWY

Theoretical pI  10.30

Acidic residues  0

Basic residues  4

Polar residues  3

Molecular weight (Average)  1699.15

Molecular weight (Monoisotopic)  1697.97

Common amino acids  AK

Net charge  4

Instability index (II)  60.42

Aliphatic index  116.25

Grand average of hydropathicity (GRAVY)  0.556

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Should not be visible by UV spectrophotometry.

Amino acid distribution



Literature Information


Literature 1

Pubmed ID Not available

Title  Structural modification and antitumor activity of antimicrobial peptide HYL

Doi 10.1016/j.cclet.2019.10.013

Year  2020

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.