Neutrophil antibiotic peptide NP-1
General Information
DCTPep ID DCTPep01920
Peptide Name Neutrophil antibiotic peptide NP-1
Sequence VTCYCRRTRCGFRERLSGACGYRGRIYRLCCR
Sequence Length 32
UniProt ID Q62716
PubChem CID Not available
Origin Rattus norvegicus
Type Native peptide
Classification
Membrane-targeted
Activity Information
Cell Line | Disease | Activity | Assay | Testing Time | Literature |
---|---|---|---|---|---|
Hep-G2 | Hepatoblastoma | 20-25% Killing=16-256µM | MTT assay | 20-24 h | 1 |
Hemolytic Activity Human erythrocytes: 5%Hemolysis>128 µM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep01920
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys3<--->Cys31; Cys5<--->Cys20; Cys10<--->Cys30
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C158H261N59O41S6
Absent amino acids DHKMNPQW
Theoretical pI 9.99
Acidic residues 1
Basic residues 9
Polar residues 16
Molecular weight (Average) 3835.54
Molecular weight (Monoisotopic) 3832.85
Common amino acids R
Net charge 8
Instability index (II) 20.14
Aliphatic index 48.75
Grand average of hydropathicity (GRAVY) -0.494
Half Life
100 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 4845
Abs 0.1% (=1 g/l) 1.263, assuming all pairs of Cys residues form cystines
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 1.165, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 29282543
Title Characterization of bactericidal efficiency, cell selectivity, and mechanism of short interspecific hybrid peptides
Year 2018
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_11174